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However, others have not found an association between maternal intake, umbilical cord blood levels, and the risk of asthma [167,168,169,170,171]

Thereafter, lungs were either frozen to isolate RNA, inflation-fixed with paraformaldehyde for histology or lavaged to collect bronchoalveolar lavage fluid ( RNA ANALYSIS Total RNA was isolated from frozen lung tissue, quantified by spectrophotometry and assayed for messenger RNA transcript abundance using standard real time PCR methodology as described ( HISTOLOGY AND CYTOLOGY Standard techniques were used to fix lung tissue in 4% paraformaldehyde and to embed in paraffin

Science 290(5493):985989 Giorgi FS, Bandettini di Poggio, A, Battaglia G, Pellegrini A, Murri L, Ruggieri S, Paparelli A and Fornai F (2006) A short overview on the role of alpha-synuclein and proteasome in experimental models of Parkinsons disease

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